Enameloplasty effects on microleakage of pit and fissure sealants under load: an in vitro study
Christina Zervou / Gerald Kugel / Cataldo Leone / Athanasios Zavras / Eileen H Doherty / George E.White

Enameloplasty has been implicated in the successful application of pit and fissure sealants.The present study aimed to evaluate the effect of enameloplasty technique on microleakage of sealants when occlusal force was applied on the teeth. The study also allowed a direct comparison of the effectiveness of enameloplasty, when two different burs were used. Six groups of ten human extracted wisdom teeth were tested. Group A: no load, conventional (no enameloplasty - control); Group B: no load, enameloplasty 1/2 round bur (Brasseler USA); Group C: no load, enameloplasty diamond fissure bur REF/UP 791( Ultradent); Group D: load 500 N, conventional , Group E: load 500 N, 1/2 round bur; Group F: load 500 N, diamond fissure bur. Specimens were thermocycled for 500 cycles at 5°C and 55°C with a dwell time of 30 seconds after load application.
Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underling fissure.The Kruskal-Wallis One Way Anova and Mann -Whitney U test showed group D having the greatest degree of microleakage statistically significant (p<0.05) comparing with other groups. Sealants prepared with the conventional technique (A, D) had statistically significant differences in microleakage with those prepared with enameloplasty (B,C,E, F) (p = 0.01). Enameloplasty in groups with no load (B, C) did reduce microleakage, but not significantly (p = 0.3) However, loaded teeth with enameloplasty appeared to perform superior, as compared to those without (p=0.005) Regarding the effect of load, teeth without load (Groups A,B, C) were found to have significantly less microleakage than teeth where mechanical force had been applied (Groups D, E, F), (p = 0.01). Microleakage in Group A (conventional technique without load) was significantly less than microleakage in Group D (conventional technique with load), (p = 0.04). However, load did not seem to influence microleakage when enameloplasty had been performed.This finding was true for both round bur enameloplasty (p = 0.29), and fissure bur enameloplasty (p = 0.26). There was no statistically significant difference between Groups B, E (round bur) and C, F (fissure bur) (p > 0.05), or between Groups B and C (p > 0.05) and between Groups E and F (p > 0.05).The results of the study indicated that enameloplasty reduced microleakage of pit and fissure sealants, especially when load was applied to teeth, irrespective of what bur was used to enlarge the fissure, as there was no statistical significant differences between the round and fissured diamond burs.The application of occlusal force to the tooth produces significantly more microleakage, unless enameloplasty is performed.