The Detection of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in tooth, tongue and buccal mucosa plaques in children, using immunoslot blot assay(IBA)
Shoji Tanaka / Maki Minami / Yukio Murakami / Takako Ogiwara / Kazuhito Seto / Masao Shoji / Atushi Hirata / Sinnosuke Abe / Sigeru Watanabe / Seiichiro Fujisawa

The present study was to investigate the distribution of typical periodontpathic bacteria (Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans) in tooth, tongue and buccal mucosa plaques in 3 to 17 Year old children. Clinical parameters (Rates of df, d, DMF, and D; plaque and gingival index) for each subject were determined prior to the collection of each site plaque. Three periodontopathic bacteria on each site samples were detected using IBA. The frequency of three bacteria for tooth plaque was higher than that for tongue or buccal mucosa plaque. The frequency of Porphyromonas gingivalis and Prevotella intermedia in supragingival plaques was significantly higher than that of corresponding ones in tongue or buccal mucosa plaques. The three bacteria also occurred more frequently in subjects aged between 10 and 14 years. Periodontopathic bacteria may be enhanced in circumpubertal children.